© 2017 Rehen Lab

Produtividade e inovação

 

 

O LaNCE destacou-se como centro gerador de conhecimento no Brasil utilizando células-tronco pluripotentes humanas como modelo experimental.

Dezenas de artigos foram publicados pela equipe em revistas científicas indexadas.

Quase 200 estudantes de diferentes regiões do Brasil, além de América Latina e Europa, já foram treinados por nossa equipe.

Com investimentos originais do BNDES, FINEP e RDSL, o LaNCE produziu e distribuiu insumos a dezenas de laboratórios que realizam pesquisa no país.

 

Patente

 

Stem cell defined media for xeno-free and feeder free conditions and uses thereof

 

Publicações Científicas e Preprints

 

2019

Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms 

Goto-Silva L, Ayad NME, Herzog IL, Silva NP, Lamien B, Orlande HRB, da Costa Souza A, Ribeiro S, Martins M, Domont GB, Junqueira M, Tovar-Moll F, Rehen SK. Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms. BMC Dev Biol. 2019 Mar 7;19(1):3.

Beyond members of the Flaviviridae family, sofosbuvir also inhibits chikungunya virus replication
André C. Ferreira, Patrícia A. Reis, Caroline S. de Freitas, Carolina Q. Sacramento, Lucas Villas Bôas Hoelz, Mônica M. Bastos, Mayara Mattos, Natasha Rocha, Isaclaudia Gomes de Azevedo Quintanilha, Carolina da Silva Gouveia Pedrosa, Leticia Rocha Quintino Souza, Erick Correia Loiola, Pablo Trindade, Yasmine Rangel Vieira, Giselle Barbosa-Lima, Hugo C. de Castro Faria Neto, Nubia Boechat, Stevens K. Rehen, Karin Brüning, Fernando A. Bozza, Patrícia T. Bozza, Thiago Moreno L. Souza

2018

Characterization of a human induced Pluripotent Stem (iPS) cell line (INCABRi002-A) derived from a primary myelofibrosis patient harboring the 5-bp insertion in CALR and the p.W146X mutation in TP53
Cintia E. Gomez Limia, Sylvie Devalle, Marcelo Reis, Jaroslaw Sochacki, Rodrigo Madeiro da Costa, Mariana D'Andrea, Telma Padilha, Ilana R. Zalcberg, Cristiana Solza, Adelmo Daumas, Stevens Rehenb, Martín H. Bonaminoa, Bárbara Monte-Mór

Human-specific approaches to brain research for the 21st century: a South American perspective
Marcia Triunfol, Stevens Rehen, Marina Simian, Troy Seidle

Specific cytostatic and cytotoxic effect of dihydrochelerythrine in glioblastoma cells: role of NF-kB/b-catenin and STAT3/IL-6 pathways
Tereza Cristina Costa Silva, Noelio de Jesus Menezes-Filho, Giselle Pinto de Faria Lopes, Diego Madureira de Oliveira, Ezequiel Pereira, Bruno Penas Seara Pitanga, Eudes S Velozo, Songeli Menezes Freire, Jorge Clarencio de Souza Andrade, Helena Lobo Borges, Stevens Kastrup Rehen, Vivaldo Moura-Neto, Silvia Lima Costa

Deformation of Mitochondrial Cristae in Human Neural Progenitor Cells Exposed to Valproic Acid.
Rodrigo M. da Costa, Karina Karmirian, Stevens K. Rehen

Generation Of iPSC-Derived Human Peripheral Sensory Neurons Releasing Substance P Elicited By TRPV1 Agonists
Marilia Zaluar P Guimaraes, Rodrigo De Vecchi, Gabriela Vitoria, Jaroslaw K Sochacki, Bruna Paulsen, Igor Lima, Felipe Rodrigues da Silva, Rodrigo F. Madeiro da Costa, Lionel Breton, Stevens K Rehen

 

hiPSC-derived neural stem cells from patients with schizophrenia induce an impaired angiogenesis
Bárbara S. Casas, Gabriela Vitória, Marcelo N. do Costa, Rodrigo Madeiro da Costa, Pablo Trindade, Renata Maciel, Nelson Navarrete, Stevens K. Rehen e Verónica Palma
Translational Psychiatryvolume 8, Article number: 48 (2018)

 

2017

 

Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT
Vanja Dakic, Juliana Minardi Nascimento, Rafaela Costa Sartore, Renata de Moraes Maciel, Draulio B. de Araujo, Sidarta Ribeiro, Daniel Martins-de-Souza & Stevens K. Rehen
Scientific Reports 7, Article number: 12863

Generation and characterization of a human induced pluripotent stem (iPS) cell line derived from an acute myeloid leukemia patient evolving from primary myelofibrosis carrying the CALR 52 bp deletion and the ASXL1 p.R693X mutation
Cintia E. Gomez Limia, Sylvie Devalle, Marcelo Reis, Jaroslaw Sochacki, Mayra Carneiro, Rodrigo Madeiro da Costa, Mariana D'Andrea, Telma Padilha, Ilana R. Zalcberg, Cristiana Solza, Adelmo Daumas, Stevens Rehen, Bárbara Monte-Mór, , Martín H. Bonamino.
https://doi.org/10.1016/j.scr.2017.08.006

Inhibition of pRB Pathway Differentially Modulates Apoptosis in Esophageal Cancer Cells

Soletti RC, Biasoli D, Rodrigues NALV, Delou JMA, Maciel R, Chagas VLA, Martins RAP, Rehen SK, Borges HL.

Translational Oncology 10 (5), 726-733

Combining Patient-Reprogrammed Neural Cells and Proteomics as a Model to Study Psychiatric Disorders
Zuccoli GS, Martins-de-Souza D, Guest PC, Rehen SK, Nascimento JM.
Adv Exp Med Biol. 2017;974:279-287. doi: 10.1007/978-3-319-52479-5_26.

Derivation of Functional Human Astrocytes from Cerebral Organoids
Dezonne RS, Sartore RC, Nascimento JM, Saia-Cereda VM, Romão LF, Alves-Leon SV, de Souza JM, Martins-de-Souza D, Rehen SK, Gomes FC.
Sci Rep. 2017 Mar 27;7:45091. doi: 10.1038/srep45091.

The spectrum of neuropathological changes associated with congenital Zika virus infection

Chimelli L, Melo ASO, Avvad-Portari E, Wiley CA, Camacho AHS, Lopes VS, Machado HN, Andrade CV, Dock DCA, Moreira ME, Tovar-Moll F, Oliveira-Szejnfeld PS, Carvalho ACG, Ugarte ON, Batista AGM, Amorim MMR, Melo FO, Ferreira TA, Marinho JRL, Azevedo GS, Leal JIBF, da Costa RFM, Rehen S, Arruda MB, Brindeiro RM, Delvechio R, Aguiar RS, Tanuri A.
Acta Neuropathol. 2017 Jun;133(6):983-999. doi: 10.1007/s00401-017-1699-5. Epub 2017 Mar 22.

 

Trace elements during primordial plexiform network formation in human cerebral organoids
Rafaela C. Sartore, Simone C. Cardoso, Yury V.M. Lages, Julia M. Paraguassu, Mariana P. Stelling, Rodrigo F. Madeiro da Costa, Marilia Z. Guimaraes, Carlos A. Pérez, Stevens K. Rehen
PeerJ, 2017

Zika virus disrupts molecular fingerprinting of human neurospheres
PP Garcez, JM Nascimento, JM de Vasconcelos, RM da Costa, ...
Scientific Reports 7, 40780, 2017

The clinically approved antiviral drug sofosbuvir inhibits Zika virus replication
CQ Sacramento, GR de Melo, CS de Freitas, N Rocha, LVB Hoelz, ...
Scientific Reports 7, 40920, 2017

 

2016

Harmine stimulates proliferation of human neural progenitors
V Dakic, R de Moraes Maciel, H Drummond, JM Nascimento, P Trindade, ...
PeerJ 4, e2727, 2016

Chloroquine, an Endocytosis Blocking Agent, Inhibits Zika Virus Infection in Different Cell Models
R Delvecchio, LM Higa, P Pezzuto, AL Valadão, PP Garcez, FL Monteiro, ...
Viruses 8 (12), 322, 2016

Generation of urine iPS cell lines from patients with Attention Deficit Hyperactivity Disorder (ADHD) using a non-integrative method
Jaroslaw Sochacki, Sylvie Devalle, Marcelo Reis, Paulo Mattos, Stevens Rehen
Stem Cell Research, 2016

 

Generation of urine iPS cell line from a patient with obsessive-compulsive disorder using a non-integrative method
Jaroslaw Sochacki, Sylvie Devalle, Marcelo Reis, Leonardo F Fontenelle, Stevens Rehen
Stem Cell Research, 2016

 

Generation of iPS cell lines from schizophrenia patients using a non-integrative method
Jaroslaw Sochacki, Sylvie Devalle, Marcelo Reis, Renata Maciel, Bruna Paulsen, Helena Brentani, Paulo Silva Belmonte-de-Abreu, Stevens Rehen
Stem Cell Research, 2016

Zika virus impairs growth in human neurospheres and brain organoids
PP Garcez, EC Loiola, RM da Costa, LM Higa, P Trindade, R Delvecchio, ...
Science 352 (6287), 816-818

Combined proteome and transcriptome analyses reveal that Zika virus circulating in Brazil alters cell cycle and neurogenic programmes in human neurospheres
Patricia P Garcez, Juliana Minardi Nascimento, Janaina Mota de Vasconcelos, Rodrigo Madeiro da Costa, Rodrigo Delvecchio, Pablo Trindade, Erick Correia Loiola, Luiza M Higa, Juliana Cassoli, Gabriela Vitória, Patricia Sequeira, Jaroslaw Sochacki, Renato S Aguiar, Hellen Thais Fuzii, Ana M Bispo de Filippis, João Lídio da Silva Gonçalves Vianez, Daniel Martins-de-Souza, Amilcar Tanuri, Stevens K Rehen
PeerJ Preprints, 2016

 

Chloroquine inhibits Zika Virus infection in different cellular models
Rodrigo Delvecchio, Luiza M Higa, Paula Pezzuto, Ana Luiza Valadao, Patricia P Garcez, Fabio L Monteiro, Erick C Loiola, Stevens Rehen, Loraine Campanati, Renato Santana de Aguiar, Amilcar Tanuri
bioRxiv Preprint, 2016

 

2015

 

Low oxygen alters mitochondrial function and response to oxidative stress in human neural progenitor cells
Yury M. Lages, Juliana M. Nascimento, Gabriela A. Lemos, Antonio Galina, Leda R. Castilho, Stevens K. Rehen​
Peer J, 2015

Valproate Disturbs Morphology and Mitochondrial Membrane Potential in Human Neural Cells
RFM da Costa, ML Kormann, A Galina, SK Rehen
Applied In Vitro Toxicology 1 (4), 254-261, 2015

Stem cell research in Latin America: update, challenges and opportunities in a priority research area
V Palma, FJ Pitossi, SK Rehen, C Touriño, I Velasco
Regenerative medicine 10 (06), 785-798, 2015

Co-expression network of neural-differentiation genes shows specific pattern in schizophrenia
M Maschietto, AC Tahira, R Puga, L Lima, D Mariani, ...
BMC medical genomics 8 (1), 23, 2015

Mitomycin-treated undifferentiated embryonic stem cells as a safe and effective therapeutic strategy in a mouse model of Parkinson’s disease
Mariana Acquarone, Thiago M. de Melo, Fernanda Meireles, Jordano Brito-Moreira, Gabriel Oliveira, Sergio T. Ferreira, Newton G. Castro, Fernanda Tovar-Moll, Jean-Christophe Houzel and Stevens K. Rehen
Front. Cell. Neurosci., 2015

Reprogramming human cells to study mental disorders
S Rehen
SCHIZOPHRENIA BULLETIN 41, S116-S116, 2015

Genomic mosaicism with increased amyloid precursor protein (APP) gene copy number in single neurons from sporadic Alzheimer's disease brains
Diane M Bushman, Gwendolyn E Kaeser, Benjamin Siddoway, Jurgen W Westra, Richard R Rivera, Stevens K Rehen, Yun C Yung, Jerold Chun
eLife, 2015

 

Commitment of human pluripotent stem cells to a neural lineage is induced by the pro-estrogenic flavonoid apigenin
CS Souza, BS Paulsen, S Devalle, S Lima Costa, HL Borges, SK Rehen
Advances in Regenerative Biology 2 (1), 29244, 2015

2014

Accelerating neuronal aging in in vitro model brain disorders: a focus on reactive oxygen species
PB Campos, BS Paulsen, SK Rehen
Frontiers in aging neuroscience 6, 292, 2014

Glioblastoma cells inhibit astrocytic p53-expression favoring cancer malignancy
D Biasoli, MF Sobrinho, ACC Da Fonseca, DG De Matos, L Romão, ...
Oncogenesis 3 (10), e123, 2014

 

LPA-primed astrocytes induce axonal outgrowth of cortical progenitors by activating PKA signaling pathways and modulating extracellular matrix proteins
Spohr TC, Dezonne RS, Rehen SK, Gomes FC.
Front Cell Neurosci. 2014

Reprogramming cells to study mental disorders

S Rehen

BRAIN PATHOLOGY 24, 30-30, 2014

Valproate reverts zinc and potassium imbalance in schizophrenia-derived reprogrammed cells
Bruna da Silveira Paulsen, Simone Coutinho Cardoso, Mariana Paranhos Stelling, Daniel Veloso Cadilhe, Stevens Kastrup Rehen
Schizophrenia Research, 2014

Murine dopaminergic Müller cells restore motor function in a model of Parkinson's disease
B Stutz, FSL Conceição, LE Santos, DV Cadilhe, RL Fleming, ...
Journal of neurochemistry 128 (6), 829-840, 2014

Murine dopaminergic Müller cells restore motor function in a model of Parkinson's disease | NOVA. The University of Newcastle's Digital Repository
B Stutz, FS Lima da Conceição, S Rehen, JC Houzel, FG de Mello, ...
Wiley-Blackwell, 2014

2013

2,4-dinitrophenol induces neural differentiation of murine embryonic stem cells
Léo Freitas-Correa, Mychael V. Lourenco, Mariana Acquarone, Rodrigo F. Madeiro da Costa, Antonio Galina, Stevens K. Rehen, Sergio T. Ferreira
Stem Cell Research, 2013

 

Pluripotent stem cells as a model to study oxygen metabolism in neurogenesis and neurodevelopmental disorders
Paulsen BD, Silveira MS, Galina A, Rehen SK.
Arch Biochem Biophys., 2013

 

Matrix bound heparan sulfate is essential for the growth and pluripotency of human embryonic stem cells
Mariana P. Stelling; Yury M. V. Lages; Ana M. F. Tovar; Paulo A. S. Mourao; Stevens K. Rehen.
Glycobiology, 2013


2012

Aneuploid cells are differentially susceptible to caspase-mediated death during embryonic cerebral cortical development
Suzanne E. Peterson, Amy H. Yang, Diane M. Bushman, Jurjen W. Westra, Yun C. Yung, Serena Barral, Tetsuji Mutoh, Stevens K. Rehen, Jerold Chun.
The Journal of Neuroscience, 2012

Xeno-free production of human embryonic stem cells in stirred microcarrier systems using a novel animal/human-component-free medium
PAN Marinho, DT Vareschini, IC Gomes, BS Paulsen, DR Furtado, ...
Tissue Engineering Part C: Methods 19 (2), 146-155, 2012

Sphingosine 1‐phosphate‐primed astrocytes enhance differentiation of neuronal progenitor cells
TCLS Spohr, RS Dezonne, J Nones, C dos Santos Souza, ...
Journal of neuroscience research 90 (10), 1892-1902, 2012

Implications of aneuploidy for stem cell biology and brain therapeutics
Sylvie Devalle, Rafaela C. Sartore, Bruna S. Paulsen, Helena L. Borges, Rodrigo A. Martins and Stevens K. Rehen.
Frontiers in Cellular Neuroscience, 2012

Altered oxygen metabolism associated to neurogenesis of induced pluripotent stem cells derived from a schizophrenic patient
BS Paulsen, RM Maciel, A Galina, MS da Silveira, CS Souza, ...
Cell transplantation 21 (7), 1547-1559, 2012

Neural induced embryoid bodies present high levels of metals detected by x-ray microfluorescence
Mariana P. Stelling, Simone C. Cardoso, Bruna S. Paulsen e Stevens K. Rehen.
AIP Conference Proceedings, 2012

 

Cycle arrest and aneuploidy induced by zidovudine in murine embryonic stem cells
Priscila Britto Campos, Rafaela Sartore, Bia Ramalho, Elaine Costa, Stevens K. Rehen.
Mutagenesis, 2012

2011

Synchrotron Radiation X-Ray Microfluorescence Reveals Polarized Distribution of Atomic Elements during Differentiation of Pluripotent Stem Cells
Simone C. Cardoso, Mariana P. Stelling, Bruna S. Paulsen, Stevens K. Rehen.
PLoS One, 2011

 

Dynamic expression of synemin isoforms in mouse embryonic stem cells and neural derivatives
Sheila C. de Souza Martins, Agbulut O, Diguet N, Larcher JC, Bruna S. Paulsen, Stevens K. Rehen, Vivaldo Moura-Neto, Paulin D, Li Z, Xue Z.
BMC Cell Biol., 2011

Dealing with reusability and reproducibility for scientific workflows
S Lifschitz, L Gomes, SK Rehen
Bioinformatics and Biomedicine Workshops (BIBMW), 2011 IEEE International ...

Astrocytes treated by lysophosphatidic acid induce axonal outgrowth of cortical progenitors through extracellular matrix protein and epidermal growth factor signaling pathway
Tania C. E Spohr, Dezonne RS, Stevens K. Rehen, Flávia C. Gomes.
J Neurochem., 2011

P. 3. f. 004 Neurogenesis derived from induced pluripotent stem cells from skin biopsy of patients with schizophrenia and normal controls
P Belmonte-de-Abreu, B Paulsen, S Rehen, R Maciel, A Gallina, ...
European Neuropsychopharmacology 21, S525, 2011

Human dental pulp cells: A new source of cell therapy in a mouse model of compressive spinal cord injury
Fernanda Martins de Almeida, Suelen Adriani Marques, Bruna dos Santos Ramalho, Rafaela Fintelman Rodrigues, Daniel Veloso Cadilhe, Daniel Furtado, Irina Kerkis, Lygia Veiga Pereira, Stevens Karstrup Rehen, Ana Maria Blanco Martinez.

Jounal of Neurotrauma / PMID: 21609310, 2011

Normal human pluripotent stem cell lines exhibit pervasive mosaic aneuploidy
SE Peterson, JW Westra, SK Rehen, H Young, DM Bushman, ...
PLoS One 6 (8), e23018, 2011

Retinoic acid-treated pluripotent stem cells undergoing neurogenesis present increased aneuploidy and micronuclei formation
Sartore, R.C.; Campos, P.B.; Trujillo, C.A.; Ramalho, B.S.L.; Negraes, P.D.; Paulsen,  B.S.; Meletti, T.; Costa, E.S.; Chicaybam, L.; Bonamino, M.H.; Ulrich, H.; Rehen, S.K.
PLoS ONE, 2011

Functional dopaminergic differentiation of cultured Müller cells: recovery of locomotor deficits in a mouse model of Parkinson's disease
B Stutz, FSL Conceiçãc, DV Cadilhe, JC Houzel, PF Gardino, R Ram, ...
The Febs Journal 278, 276, 2011

Stem cell defined media for xeno-free and feeder free conditions and uses thereof
A Muotri, PAN Marinho, SK Rehen, LDR Castilho
US Patent App. 13/696,286, 2011

Agathisflavone enhances retinoic acid-induced neurogenesis and its receptors α and β in pluripotent stem cells
BS Paulsen, CS Souza, L Chicaybam, MH Bonamino, M Bahia, SL Costa, ...
Stem cells and development 20 (10), 1711-1721, 2011

 

Agathisflavone Enhances Retinoic Acid-Induced Neurogenesis and Its Receptors α and β in Pluripotent Stem Cells
CS Souza, L Chicaybam, MH Bonamino, MV Bahia, SL Costa, HL Borges, ...
2011

 

CT-0511 Accepted 8/29/2011 for publication in “Cell Transplantation”
B da Silveira Paulsen, R de Moraes Maciel, A Galina, MS da Silveira, ... 2011

 

A graph-mining algorithm for automatic detection and counting of embryonic stem cells in fluorescence microscopy images
GM Faustino, M Gattass, CJP de Lucena, PB Campos, SK Rehen
Integrated Computer-Aided Engineering 18 (1), 91-106, 2011

2010

Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies
Gomes IC, Acquarone M, Maciel RdM, Erlich RB, Rehen SK.
JoVE (Journal of Visualized Experiments), e2344-e2344, 2010

Predifferentiated embryonic stem cells promote functional recovery after spinal cord compressive injury
SA Marques, FM Almeida, AM Fernandes, C dos Santos Souza, ...
Brain research 1349, 115-128, 2010

Worldwide survey of published procedures to culture human embryonic stem cells
AM Fernandes, T Meletti, R Guimarães, MP Stelling, PAN Marinho, ...
Cell transplantation 19 (5), 509-523, 2010

 

Maintenance of pluripotency in mouse embryonic stem cells cultivated in stirred microcarrier cultures
PAN Marinho, AM Fernandes, JC Cruz, SK Rehen, LR Castilho
Biotechnology progress 26 (2), 548-555, 2010

Murine model for Parkinson's disease: from 6-OH dopamine lesion to behavioral test
FSL da Conceição, S Ngo-Abdalla, JC Houzel, SK Rehen
JoVE (Journal of Visualized Experiments), e1376-e1376, 2010

Aneuploidy in Embryonic Stem Cells
RC Sartore, PB Campos, MJ McConnell, SK Rehen
Perspectives of Stem Cells, 73-86, 2010

Improved Automatic ES Cells Counting Method in Fluorescence Microscopy Images
G Faustino, M Gattass, CJP de Lucena, PB Campos, S Rehen
Proceedings of International Conference on theoretical, experimental and ... 2010

Cannabinoids modulate cell survival in embryoid bodies
SK Rehen
Cell Biol. Int 34, 399-408, 2010

 

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